X-ray diffraction
1.4Å resolution

Crystal structure of a marine metagenome TRAP solute binding protein specific for pyroglutamate (Sorcerer II Global Ocean Sampling Expedition, unidentified microbe, scf7180008839099) in complex with co-purified pyroglutamate

Source organism: uncultured bacterium
Entry authors: Fedorov E, Vetting MW, Hogle SL, Dupont CL, Almo SC, Ghosh A

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
TRAP-type C4-dicarboxylate transport system, periplasmic component Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.38 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
  • Canonical: G5ZWD6 (Residues: 1-1, 24-323; Coverage: 93%)
Gene name: HIMB100_00003270
Sequence domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P61
Unit cell:
a: 56.251Å b: 56.251Å c: 193.919Å
α: 90° β: 90° γ: 120°
R R work R free
0.152 0.151 0.168
Expression system: Escherichia coli BL21(DE3)