› 6wf1

X-ray diffraction
4.19Å resolution

Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H10 Hemagglutinin

Released:
DOI: 10.2210/pdb6wf1/pdb
PDB entry 6wf1 coloured by chain, front view.
PDB entry 6wf1 coloured by chain, side view.
PDB entry 6wf1 coloured by chain, top view.
Source organisms:
Primary publication:
Unique structural solution from a VH3-30 antibody targeting the hemagglutinin stem of influenza A viruses.
Harshbarger WD, Deming D, Lockbaum GJ, Attatippaholkun N, Kamkaew M, Hou S, Somasundaran M, Wang JP, Finberg RW, Zhu QK, Schiffer CA, Marasco WA
Nat Commun 12 559 (2021)
PMID: 33495478

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207211 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.4 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A059T4A1 (Residues: 18-340; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 25.09 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A059T4A1 (Residues: 341-514; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
heavy chain Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P321
Unit cell:
a: 127.021Å b: 127.021Å c: 158.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.261 0.308
Expression system: Trichoplusia ni

Quick links

Citations

3 review citations

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
2 more

1 mention without citation

Unique structural solution from a VH3-30 antibody targeting the hemagglutinin stem of influenza A viruses.
Harshbarger et al. (2021)