› 6wf1

X-ray diffraction
4.19Å resolution

Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H10 Hemagglutinin

Released:
DOI: 10.2210/pdb6wf1/pdb
PDB 6wf1 coloured by chain and viewed from the front.
PDB 6wf1 coloured by chain and viewed from the side.
PDB 6wf1 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Unique structural solution from a VH3-30 antibody targeting the hemagglutinin stem of influenza A viruses.
Harshbarger WD, Deming D, Lockbaum GJ, Attatippaholkun N, Kamkaew M, Hou S, Somasundaran M, Wang JP, Finberg RW, Zhu QK, Schiffer CA, Marasco WA
Nat Commun 12 559 (2021)
PMID: 33495478

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.4 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A059T4A1 (Residues: 18-340; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 25.09 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A059T4A1 (Residues: 341-514; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
heavy chain Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P321
Unit cell:
a: 127.021Å b: 127.021Å c: 158.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.261 0.308
Expression system: Trichoplusia ni

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Citations

1 review citation

Protein engineering strategies for rational immunogen design.
Caradonna et al. (2021)
2 other articles