› 6wdn

Electron Microscopy
3.2Å resolution

Cryo-EM structure of mitochondrial calcium uniporter holocomplex in low Ca2+

Released:
DOI: 10.2210/pdb6wdn/pdb
PDB entry 6wdn coloured by chain, front view.
PDB entry 6wdn coloured by chain, side view.
PDB entry 6wdn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and mechanism of the mitochondrial Ca2+ uniporter holocomplex.
Fan M, Zhang J, Tsai CW, Orlando BJ, Rodriguez M, Xu Y, Liao M, Tsai MF, Feng L
Nature 582 129-133 (2020)
PMID: 32494073
Related structures: EMD-21642

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-184951 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Calcium uptake protein 2, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 39.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8IYU8 (Residues: 84-418; Coverage: 77%)
Gene names: EFHA1, MICU2
Calcium uptake protein 1, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 363 amino acids
Theoretical weight: 41.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BPX6 (Residues: 104-466; Coverage: 76%)
Gene names: CALC, CBARA1, MICU1
Sequence domains: EF hand
Essential MCU regulator, mitochondrial Chains: D, F, H, J
Molecule details ›
Chains: D, F, H, J
Length: 51 amino acids
Theoretical weight: 5.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H4I9 (Residues: 48-98; Coverage: 48%)
Gene names: C22orf32, EMRE, SMDT1
Sequence domains: Putative mitochondrial precursor protein
Calcium uniporter protein, mitochondrial Chains: C, E, G, I
Molecule details ›
Chains: C, E, G, I
Length: 178 amino acids
Theoretical weight: 21.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NE86 (Residues: 169-346; Coverage: 51%)
Gene names: C10orf42, CCDC109A, MCU
Sequence domains: Mitochondrial calcium uniporter

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-21642
Expression system: Homo sapiens

Quick links

Citations

36 review citations

The Mitochondrial Ca2+ Uptake and the Fine-Tuning of Aerobic Metabolism.
Gherardi et al. (2020)
35 more

9 mentions without citation

Molecular nature and physiological role of the mitochondrial calcium uniporter channel.
Alevriadou et al. (2021)
8 more