› 6w9e

X-ray diffraction
3.1Å resolution

Crystal Structure of Human CDK9/cyclinT1 in complex with MC180295

Released:
DOI: 10.2210/pdb6w9e/pdb
PDB entry 6w9e coloured by chain, front view.
PDB entry 6w9e coloured by chain, side view.
PDB entry 6w9e coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zhang P, Wu J

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130021 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 9 Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 38.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P50750 (Residues: 1-330; Coverage: 89%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Cyclin-T1 Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 30.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O60563 (Residues: 1-259; Coverage: 36%)
Gene name: CCNT1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand TO7 2 x TO7
Ligand TOJ 2 x TOJ
Ligand PO4 1 x PO4
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: R3
Unit cell:
a: 173.34Å b: 173.34Å c: 97.368Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.172 0.221
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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