6w8z

X-ray diffraction
1.9Å resolution

Co-crystal structures of CHIKV nsP3 macrodomain with pyrimidone fragments

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate
ADP-D-ribose 1''-phosphate + H(2)O = ADP-D-ribose + phosphate
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 168 amino acids
Theoretical weight: 18.52 KDa
Source organism: Chikungunya virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8JUX6 (Residues: 1334-1493; Coverage: 7%)
Sequence domains: Macro domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P31
Unit cell:
a: 87.676Å b: 87.676Å c: 83.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.256
Expression system: Escherichia coli BL21(DE3)