› 6w78

X-ray diffraction
2.31Å resolution

crystal structure of a plant ice-binding protein

Released:
DOI: 10.2210/pdb6w78/pdb
PDB 6w78 coloured by chain and viewed from the front.
PDB 6w78 coloured by chain and viewed from the side.
PDB 6w78 coloured by chain and viewed from the top.
Source organism: Daucus carota
Primary publication:
Carrot 'antifreeze' protein has an irregular ice-binding site that confers weak freezing point depression but strong inhibition of ice recrystallization.
Wang Y, Graham LA, Han Z, Eves R, Gruneberg AK, Campbell RL, Zhang H, Davies PL
Biochem J 477 2179-2192 (2020)
PMID: 32459306

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LRRNT_2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 37.16 KDa
Source organism: Daucus carota
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O82438 (Residues: 1-332; Coverage: 100%)
Gene names: AFP, afp
Sequence domains: Leucine rich repeat

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 38.895Å b: 57.453Å c: 135.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.223
Expression system: Trichoplusia ni

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