6w75

X-ray diffraction
1.95Å resolution

1.95 Angstrom Resolution Crystal Structure of NSP10 - NSP16 Complex from SARS-CoV-2

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H(2)O = ADP + phosphate
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2'-O-methyltransferase nsp16 Chains: A, C
Molecule details ›
Chains: A, C
Length: 301 amino acids
Theoretical weight: 33.63 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DTD1 (Residues: 6799-7096; Coverage: 4%)
Gene names: 1a-1b, rep
Sequence domains: Coronavirus 2'-O-methyltransferase
Non-structural protein 10 Chains: B, D
Molecule details ›
Chains: B, D
Length: 142 amino acids
Theoretical weight: 15.08 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DTD1 (Residues: 4254-4392; Coverage: 2%)
Gene names: 1a-1b, rep
Sequence domains: Coronavirus RNA synthesis protein NSP10

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 166.245Å b: 166.245Å c: 98.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.157 0.174
Expression system: Escherichia coli BL21(DE3)