› 6w6n

X-ray diffraction
2.25Å resolution

K106L/A131E mutant of cytochrome P460 from Nitrosomonas sp. AL212

Released:
DOI: 10.2210/pdb6w6n/pdb
PDB 6w6n coloured by chain and viewed from the front.
PDB 6w6n coloured by chain and viewed from the side.
PDB 6w6n coloured by chain and viewed from the top.
Source organism: Nitrosomonas sp. AL212
Primary publication:
The Heme-Lys Cross-Link in Cytochrome P460 Promotes Catalysis by Enforcing Secondary Coordination Sphere Architecture.
Coleman RE, Vilbert AC, Lancaster KM
Biochemistry 59 2289-2298 (2020)
PMID: 32525655

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome_P460 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 17.91 KDa
Source organism: Nitrosomonas sp. AL212
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F9ZFJ0 (Residues: 35-194; Coverage: 95%)
Gene name: NAL212_0896
Sequence domains: Cytochrome P460

Ligands and Environments

1 bound ligand:
Ligand HEC 2 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 47.373Å b: 80.172Å c: 110.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.258 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

A novel catalytic heme cofactor in SfmD with a single thioether bond and a bis-His ligand set revealed by a de novo crystal structural and spectroscopic study.
Shin et al. (2021)
1 more