6w4e

Solution NMR

NMR-driven structure of KRAS4B-GTP homodimer on a lipid bilayer nanodisc

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Truncated apolipoprotein A-I Chains: A, D
Molecule details ›
Chains: A, D
Length: 200 amino acids
Theoretical weight: 23.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P02647 (Residues: 68-265; Coverage: 80%)
Gene name: APOA1
Sequence domains: Apolipoprotein A1/A4/E domain
GTPase KRas Chains: B, C
Molecule details ›
Chains: B, C
Length: 185 amino acids
Theoretical weight: 21.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P01116 (Residues: 2-186; Coverage: 97%)
  • Best match: P01116-2 (Residues: 2-185)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 1%
Refinement method: simulated annealing
Chemical shifts: BMR30734  
Expression system: Escherichia coli K-12