› 6w41

X-ray diffraction
3.08Å resolution

Crystal structure of SARS-CoV-2 receptor binding domain in complex with human antibody CR3022

Released:
DOI: 10.2210/pdb6w41/pdb
PDB 6w41 coloured by chain and viewed from the front.
PDB 6w41 coloured by chain and viewed from the side.
PDB 6w41 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV.
Yuan M, Wu NC, Zhu X, Lee CD, So RTY, Lv H, Mok CKP, Wilson IA
Science 368 630-633 (2020)
PMID: 32245784

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CR3022 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CR3022 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 221 amino acids
Theoretical weight: 24.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Spike protein S1 Chain: C
Molecule details ›
Chain: C
Length: 231 amino acids
Theoretical weight: 26.1 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0DTC2 (Residues: 319-541; Coverage: 18%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P4122
Unit cell:
a: 147.535Å b: 147.535Å c: 200.156Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.243
Expression systems:
  • Homo sapiens
  • Trichoplusia ni

Quick links

Citations

70 review citations

Antibodies at work in the time of severe acute respiratory syndrome coronavirus 2.
Sajna et al. (2021)
69 more