› 6w2c

X-ray diffraction
6.3Å resolution

Anomalous iodine signal reveals the position of I-paroxetine complexed with the serotonin transporter at the central site

Released:
DOI: 10.2210/pdb6w2c/pdb
PDB entry 6w2c coloured by chain, front view.
PDB entry 6w2c coloured by chain, side view.
PDB entry 6w2c coloured by chain, top view.
Source organisms:
Primary publication:
Chemical and structural investigation of the paroxetine-human serotonin transporter complex.
Coleman JA, Navratna V, Antermite D, Yang D, Bull JA, Gouaux E
Elife 9 (2020)
PMID: 32618269

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211043 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium-dependent serotonin transporter Chain: A
Molecule details ›
Chain: A
Length: 549 amino acids
Theoretical weight: 61.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31645 (Residues: 76-618; Coverage: 86%)
Gene names: HTT, SERT, SLC6A4
Sequence domains: Sodium:neurotransmitter symporter family
8B6 heavy chain antibody fragment Chain: B
Molecule details ›
Chain: B
Length: 229 amino acids
Theoretical weight: 24.85 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
8B6 light chain antibody fragment Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 23.72 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand RFY 1 x RFY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 126.99Å b: 159.95Å c: 140.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.309 0.306 0.37
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

10 review citations

Highlighting membrane protein structure and function: A celebration of the Protein Data Bank.
Li et al. (2021)
9 more

2 mentions without citation

Chemical and structural investigation of the paroxetine-human serotonin transporter complex.
Coleman et al. (2020)
1 more