› 6w2c

X-ray diffraction
6.3Å resolution

Anomalous iodine signal reveals the position of I-paroxetine complexed with the serotonin transporter at the central site

Released:
DOI: 10.2210/pdb6w2c/pdb
PDB 6w2c coloured by chain and viewed from the front.
PDB 6w2c coloured by chain and viewed from the side.
PDB 6w2c coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Chemical and structural investigation of the paroxetine-human serotonin transporter complex.
Coleman JA, Navratna V, Antermite D, Yang D, Bull JA, Gouaux E
Elife 9 (2020)
PMID: 32618269

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium-dependent serotonin transporter Chain: A
Molecule details ›
Chain: A
Length: 549 amino acids
Theoretical weight: 61.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31645 (Residues: 76-618; Coverage: 86%)
Gene names: HTT, SERT, SLC6A4
Sequence domains: Sodium:neurotransmitter symporter family
8B6 heavy chain antibody fragment Chain: B
Molecule details ›
Chain: B
Length: 229 amino acids
Theoretical weight: 24.85 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
8B6 light chain antibody fragment Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 23.72 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand RFY 1 x RFY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 126.99Å b: 159.95Å c: 140.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.309 0.306 0.37
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

5 review citations

Putting on molecular weight: Enabling cryo-EM structure determination of sub-100-kDa proteins.
Wentinck et al. (2022)
4 more

2 mentions without citation

Chemical and structural investigation of the paroxetine-human serotonin transporter complex.
Coleman et al. (2020)
1 more