› 6vzq

X-ray diffraction
3.08Å resolution

Engineered TTLL6 mutant bound to alpha-elongation analog

Released:
DOI: 10.2210/pdb6vzq/pdb
PDB entry 6vzq coloured by chain, front view.
PDB entry 6vzq coloured by chain, side view.
PDB entry 6vzq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.
Mahalingan KK, Keith Keenan E, Strickland M, Li Y, Liu Y, Ball HL, Tanner ME, Tjandra N, Roll-Mecak A
Nat Struct Mol Biol 27 802-813 (2020)
PMID: 32747782

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tubulin polyglutamylase TTLL6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 453 amino acids
Theoretical weight: 53.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A4Q9E8 (Residues: 51-503; Coverage: 55%)
Gene name: Ttll6
Sequence domains: Tubulin-tyrosine ligase family

Ligands and Environments

5 bound ligands:
Ligand ADP 4 x ADP
Ligand RZY 1 x RZY
Ligand MG 8 x MG
Ligand GOL 18 x GOL
Ligand RZP 3 x RZP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 75.038Å b: 109.491Å c: 171.7Å
α: 90° β: 90.014° γ: 90°
R-values:
R R work R free
0.228 0.226 0.259
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Evolution of glutamatergic signaling and synapses.
Moroz et al. (2021)
5 more