6vw7

X-ray diffraction
2Å resolution

Formate Dehydrogenase FdsABG subcomplex FdsBG from C. necator - NADH bound

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent formate dehydrogenase gamma subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 176 amino acids
Theoretical weight: 18.75 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0KDY3 (Residues: 1-176; Coverage: 100%)
Gene names: H16_A0640, fdsG
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH_4Fe-4S domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 520 amino acids
Theoretical weight: 55.29 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0KDY2 (Residues: 1-520; Coverage: 100%)
Gene names: H16_A0641, fdsB
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI

Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2, APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 132.656Å b: 127.05Å c: 99.84Å
α: 90° β: 123.5° γ: 90°
R-values:
R R work R free
0.16 0.159 0.199
Expression system: Escherichia coli