6vw1

X-ray diffraction
2.68Å resolution

Structure of SARS-CoV-2 chimeric receptor-binding domain complexed with its receptor human ACE2

Released:

Function and Biology Details

Reaction catalysed:
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Processed angiotensin-converting enzyme 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 597 amino acids
Theoretical weight: 69.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BYF1 (Residues: 19-615; Coverage: 76%)
Gene names: ACE2, UNQ868/PRO1885
Sequence domains: Angiotensin-converting enzyme
Spike protein S1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 217 amino acids
Theoretical weight: 24.33 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0DTC2 (Residues: 455-518; Coverage: 5%)
  • Canonical: P59594 (Residues: 306-441, 505-521; Coverage: 12%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 80.435Å b: 118.034Å c: 112.075Å
α: 90° β: 93.12° γ: 90°
R-values:
R R work R free
0.198 0.197 0.229
Expression system: Spodoptera frugiperda