6vtb

X-ray diffraction
1.55Å resolution

Naegleria gruberi RNA ligase K326A mutant with ATP and Mn

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA_ligase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.6 KDa
Source organism: Naegleria gruberi
Expression system: Escherichia coli
UniProt:
  • Canonical: D2W2Z5 (Residues: 1-339; Coverage: 100%)
Gene name: NAEGRDRAFT_82186
Sequence domains: RNA ligase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 53.031Å b: 64.221Å c: 101.888Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.195
Expression system: Escherichia coli