› 6vs2

X-ray diffraction
3Å resolution

protein D

Released:
DOI: 10.2210/pdb6vs2/pdb
PDB entry 6vs2 coloured by chain, front view.
PDB entry 6vs2 coloured by chain, side view.
PDB entry 6vs2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and mechanism of a redesigned multidrug transporter from the Major Facilitator Superfamily.
Wu HH, Symersky J, Lu M
Sci Rep 10 3949 (2020)
PMID: 32127561

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug transporter MdfA Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 41.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEY8 (Residues: 14-400; Coverage: 94%)
Gene names: JW0826, b0842, cmlA, cmr, mdfA
Sequence domains: Sugar (and other) transporter

Ligands and Environments

2 bound ligands:
Ligand LDA 1 x LDA
Ligand PR 1 x PR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 95.014Å b: 66.973Å c: 110.433Å
α: 90° β: 111.1° γ: 90°
R-values:
R R work R free
0.284 0.28 0.299
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Host - Bacterial Pathogen Communication: The Wily Role of the Multidrug Efflux Pumps of the MFS Family.
Pasqua et al. (2021)
1 more

2 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
1 more