6vo5

X-ray diffraction
1.6Å resolution

Crystal structure of Human histone acetytransferas 1 (HAT1) in complex with isobutryl-COA and K12A mutant variant of histone H4

Released:
Source organism: Homo sapiens
Entry authors: Halabelian L, Zeng H, Dong A, Loppnau P, Bountra C, Edwards AM, Arrowsmith CH, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone acetyltransferase type B catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 38.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14929 (Residues: 20-341; Coverage: 77%)
  • Best match: O14929-2 (Residues: 1-256)
Gene names: HAT1, KAT1
Sequence domains: Histone acetyl transferase HAT1 N-terminus
Histone H4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 amino acids
Theoretical weight: 1.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P62805 (Residues: 2-21; Coverage: 19%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4

Ligands and Environments


Cofactor: Ligand CO6 2 x CO6
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 116.726Å b: 155.636Å c: 53.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.198
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided