› 6vjf

X-ray diffraction
2.47Å resolution

The P-Loop K to A mutation of C. therm Vps1 GTPase-BSE

Released:
DOI: 10.2210/pdb6vjf/pdb
PDB 6vjf coloured by chain and viewed from the front.
PDB 6vjf coloured by chain and viewed from the side.
PDB 6vjf coloured by chain and viewed from the top.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.
Tornabene BA, Varlakhanova NV, Hosford CJ, Chappie JS, Ford MGJ
Protein Sci 29 1416-1428 (2020)
PMID: 31981262

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative sorting protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 391 amino acids
Theoretical weight: 42.94 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G0SFF0 (Residues: 1-351, 364-372; Coverage: 52%)
Gene name: CTHT_0061810
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GCP 4 x GCP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 81.873Å b: 119.032Å c: 84.576Å
α: 90° β: 100.95° γ: 90°
R-values:
R R work R free
0.191 0.188 0.238
Expression system: Escherichia coli BL21

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Citations

2 citation in other articles

Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.
Tornabene et al. (2020)
1 more