6v99

X-ray diffraction
2.29Å resolution

Agrobacterium tumefaciens ADP-Glucose pyrophosphorylase- S72D in the presence of sulfate

Released:
Entry authors: Zheng Y, Alghamdi MA, Ballicora MA, Liu D

Function and Biology Details

Reaction catalysed:
ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate adenylyltransferase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, T, W
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, T, W
Length: 440 amino acids
Theoretical weight: 49.3 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U8L5 (Residues: 1-420; Coverage: 100%)
Gene names: AGR_L_1560, Atu4076, glgC
Sequence domains: Nucleotidyl transferase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 93.416Å b: 141.581Å c: 228.316Å
α: 108.067° β: 101.846° γ: 89.998°
R-values:
R R work R free
0.211 0.208 0.254
Expression system: Escherichia coli BL21(DE3)