› 6v7x

X-ray diffraction
2.9Å resolution

Structure of a phage-encoded quorum sensing anti-activator, Aqs1 bound to LasR

Released:
DOI: 10.2210/pdb6v7x/pdb
PDB entry 6v7x coloured by chain, front view.
PDB entry 6v7x coloured by chain, side view.
PDB entry 6v7x coloured by chain, top view.
Source organisms:
Primary publication:
A phage-encoded anti-activator inhibits quorum sensing in Pseudomonas aeruginosa.
Shah M, Taylor VL, Bona D, Tsao Y, Stanley SY, Pimentel-Elardo SM, McCallum M, Bondy-Denomy J, Howell PL, Nodwell JR, Davidson AR, Moraes TF, Maxwell KL
Mol Cell 81 571-583.e6 (2021)
PMID: 33412111

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106511 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
QUORUM SENSING ANTI-ACTIVATOR PROTEIN AQS1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 69 amino acids
Theoretical weight: 7.65 KDa
Source organism: Casadabanvirus DMS3
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0SML3 (Residues: 1-69; Coverage: 100%)
Gene name: DMS3-3
Transcriptional activator protein LasR Chain: B
Molecule details ›
Chain: B
Length: 239 amino acids
Theoretical weight: 26.65 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25084 (Residues: 1-239; Coverage: 100%)
Gene names: PA1430, lasR
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand OHN 1 x OHN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41212
Unit cell:
a: 104.536Å b: 104.536Å c: 102.601Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.249 0.281
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Digging into the lesser-known aspects of CRISPR biology.
Guzmán et al. (2021)
11 more

5 mentions without citation

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria.
Kumar et al. (2022)
4 more