X-ray diffraction
1.16Å resolution

Crystal structure of RhoA-GDP with novel Switch I conformation

Source organism: Homo sapiens
Primary publication:
Structure of an inactive conformation of GTP-bound RhoA GTPase.
Structure 29 553-563.e5 (2021)
PMID: 33497604

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 32.216Å b: 87.192Å c: 34.271Å
α: 90° β: 95.06° γ: 90°
R R work R free
0.17 0.17 0.185
Expression system: Escherichia coli BL21(DE3)