6v65

X-ray diffraction
2.76Å resolution

Crystal structure of KRAS(GMPPNP)-NF1(GRD)-SPRED1 complex

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sprouty-related, EVH1 domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.85 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q7Z699 (Residues: 13-125; Coverage: 26%)
Gene name: SPRED1
Neurofibromin truncated Chain: B
Molecule details ›
Chain: B
Length: 329 amino acids
Theoretical weight: 37.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21359 (Residues: 1203-1551; Coverage: 12%)
  • Best match: P21359-2 (Residues: 1203-1530)
Gene name: NF1
Sequence domains: GTPase-activator protein for Ras-like GTPase
GTPase KRas, N-terminally processed Chain: C
Molecule details ›
Chain: C
Length: 170 amino acids
Theoretical weight: 19.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 1-169)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 72.556Å b: 70.553Å c: 80.311Å
α: 90° β: 99.61° γ: 90°
R-values:
R R work R free
0.222 0.221 0.241
Expression systems:
  • unidentified baculovirus
  • Escherichia coli