› 6v4a

X-ray diffraction
3.83Å resolution

An open conformation of a Pentameic ligand-gated ion channel with additional N-terminal domain

Released:
DOI: 10.2210/pdb6v4a/pdb
PDB entry 6v4a coloured by chain, front view.
PDB entry 6v4a coloured by chain, side view.
PDB entry 6v4a coloured by chain, top view.
Source organism: Desulfofustis sp. PB-SRB1
Primary publication:
Structural basis for allosteric transitions of a multidomain pentameric ligand-gated ion channel.
Hu H, Howard RJ, Bastolla U, Lindahl E, Delarue M
Proc Natl Acad Sci U S A 117 13437-13446 (2020)
PMID: 32482881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neur_chan_LBD domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 642 amino acids
Theoretical weight: 71.73 KDa
Source organism: Desulfofustis sp. PB-SRB1
Expression system: Escherichia coli
UniProt:
  • Canonical: V4JF97 (Residues: 1-642; Coverage: 100%)
Gene name: JKP90_15375
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

2 bound ligands:
Ligand TRS 5 x TRS
Ligand P3A 5 x P3A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 141.064Å b: 116.028Å c: 169.63Å
α: 90° β: 109.17° γ: 90°
R-values:
R R work R free
0.257 0.256 0.291
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Recent Insight into Lipid Binding and Lipid Modulation of Pentameric Ligand-Gated Ion Channels.
Ananchenko et al. (2022)
4 other articles

3 mentions without citation

Molecular Simulations of Hydrophobic Gating of Pentameric Ligand Gated Ion Channels: Insights into Water and Ions.
Rao et al. (2021)
2 more