6v34

X-ray diffraction
3.15Å resolution

Crystal structure of BRAF V600E oncogenic mutant in complex with TAK-580

Released:
Source organism: Homo sapiens
Entry authors: Gonzalez Del-Pino G, Li K, Eck MJ

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 32.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15056 (Residues: 448-721; Coverage: 36%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 48.802Å b: 85.244Å c: 122.905Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.255 0.286
Expression system: Escherichia coli