6v2z

X-ray diffraction
3.16Å resolution

Crystal structure of the BRAF:MEK1 kinases in complex with AMPPNP and Cobimetinib

Released:
Source organism: Homo sapiens
Entry authors: Li K, Gonzalez Del-Pino G, Park E, Eck MJ

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase B-raf Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.1 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15056 (Residues: 445-723; Coverage: 36%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Dual specificity mitogen-activated protein kinase kinase 1 Chain: B
Molecule details ›
Chain: B
Length: 397 amino acids
Theoretical weight: 43.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02750 (Residues: 1-393; Coverage: 100%)
Gene names: MAP2K1, MEK1, PRKMK1
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 116.144Å b: 116.144Å c: 129.886Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.224
Expression system: Spodoptera frugiperda