6v2m

X-ray diffraction
1.66Å resolution

Structure of Escherichia coli Asp269Asn mutant phosphoenolpyruvate carboxykinase

Released:

Function and Biology Details

Reaction catalysed:
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase (ATP) Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 59.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P22259 (Residues: 1-540; Coverage: 100%)
Gene names: JW3366, b3403, pck, pckA
Sequence domains: Phosphoenolpyruvate carboxykinase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2
Unit cell:
a: 124.78Å b: 94.22Å c: 46.54Å
α: 90° β: 94.79° γ: 90°
R-values:
R R work R free
0.185 0.185 not available
Expression system: Escherichia coli