6v0o

X-ray diffraction
2.86Å resolution

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero dodecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein arginine N-methyltransferase 5 Chain: A
Molecule details ›
Chain: A
Length: 637 amino acids
Theoretical weight: 72.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O14744 (Residues: 1-637; Coverage: 100%)
Gene names: HRMT1L5, IBP72, JBP1, PRMT5, SKB1
Sequence domains:
Methylosome protein 50 Chain: B
Molecule details ›
Chain: B
Length: 342 amino acids
Theoretical weight: 36.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BQA1 (Residues: 2-342; Coverage: 100%)
Gene names: HKMT1069, MEP50, Nbla10071, WD45, WDR77
Sequence domains: WD domain, G-beta repeat
Methylosome subunit pICln Chain: D
Molecule details ›
Chain: D
Length: 13 amino acids
Theoretical weight: 1.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P54105 (Residues: 225-237; Coverage: 6%)
Gene names: CLCI, CLNS1A, ICLN

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 100.01Å b: 136.4Å c: 179.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.247
Expression systems:
  • Spodoptera frugiperda
  • Not provided