› 6uym

X-ray diffraction
2.85Å resolution

Structure of Hepatitis C Virus Envelope Glycoprotein E2mc3-v6 redesigned core from genotype 1a bound to broadly neutralizing antibody AR3C

Released:
DOI: 10.2210/pdb6uym/pdb
PDB 6uym coloured by chain and viewed from the front.
PDB 6uym coloured by chain and viewed from the side.
PDB 6uym coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Proof of concept for rational design of hepatitis C virus E2 core nanoparticle vaccines.
He L, Tzarum N, Lin X, Shapero B, Sou C, Mann CJ, Stano A, Zhang L, Nagy K, Giang E, Law M, Wilson IA, Zhu J
Sci Adv 6 eaaz6225 (2020)
PMID: 32494617

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Envelope glycoprotein E2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 176 amino acids
Theoretical weight: 19.29 KDa
Source organism: Hepatitis C virus (isolate H)
Expression system: Homo sapiens
Fab AR3C heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 233 amino acids
Theoretical weight: 24.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab AR3C light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 214 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 45.101Å b: 90.84Å c: 94.033Å
α: 84.5° β: 78.11° γ: 77.09°
R-values:
R R work R free
0.217 0.214 0.26
Expression system: Homo sapiens

Quick links

Citations

10 review citations

Progress in the Development of Structure-Based Vaccines.
Thomas et al. (2022)
9 more