X-ray diffraction
1.5Å resolution

E. coli cysteine desulfurase SufS with a spontaneously rotated beta-hairpin


Function and Biology Details

Reactions catalysed:
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 44.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P77444 (Residues: 1-406; Coverage: 100%)
Gene names: JW1670, b1680, csdB, sufS, ynhB
Sequence domains: Aminotransferase class-V

Ligands and Environments

Cofactor: Ligand PLP 2 x PLP
No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 60.54Å b: 115.74Å c: 63.66Å
α: 90° β: 114.47° γ: 90°
R R work R free
0.197 0.197 0.221
Expression system: Escherichia coli BL21(DE3)