› 6uss

X-ray diffraction
2.5Å resolution

Catalytic S88C mutant of gut microbial sulfatase from Bacteroides fragilis CAG:558

Released:
DOI: 10.2210/pdb6uss/pdb
PDB entry 6uss coloured by chain, front view.
PDB entry 6uss coloured by chain, side view.
PDB entry 6uss coloured by chain, top view.
Source organism: Bacteroides fragilis CAG:558
Primary publication:
Structural Insights into Endobiotic Reactivation by Human Gut Microbiome-Encoded Sulfatases.
Ervin SM, Simpson JB, Gibbs ME, Creekmore BC, Lim L, Walton WG, Gharaibeh RZ, Redinbo MR
Biochemistry 59 3939-3950 (2020)
PMID: 32993284

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfatase N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 517 amino acids
Theoretical weight: 57.87 KDa
Source organism: Bacteroides fragilis CAG:558
Expression system: Escherichia coli
UniProt:
  • Canonical: R5RYH7 (Residues: 1-517; Coverage: 100%)
Gene name: BN707_01784
Sequence domains: Sulfatase

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 122.633Å b: 180.378Å c: 123.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.24
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Role of Uptake and Efflux Transporters in the Disposition of Glucuronide and Sulfate Conjugates.
Järvinen et al. (2021)
6 more

1 mention without citation

Purification, Characterization, and Structural Studies of a Sulfatase from Pedobacter yulinensis.
Schlachter et al. (2021)