6urb

X-ray diffraction
2.1Å resolution

Pseudomonas aeruginosa HasA mutant - H32A

Released:
Source organism: Pseudomonas aeruginosa
Entry authors: Brimberry M, Lanzilotta W, Wilks A, Dent A

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme acquisition protein HasA Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 20.41 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: O69756 (Residues: 1-200; Coverage: 98%)
Gene names: IPC123_06085, IPC1333_05680, IPC1335_02085, IPC1488_02250, IPC1489_14710, IPC1490_04890, IPC1492_06015, IPC1494_05345, IPC1523_27745, IPC1583_01210, IPC1588_04815, IPC1594_05930, IPC1595_29025, IPC168_02755, IPC48_05880, IPC607_00315, IPC737_16450, hasAp
Sequence domains: Heme-binding protein A (HasA)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 111.34Å b: 51.867Å c: 72.811Å
α: 90° β: 130.67° γ: 90°
R-values:
R R work R free
0.236 0.233 0.302
Expression system: Escherichia coli