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X-ray diffraction
3.3Å resolution

Crystal structure of R173A variant of cytosolic fumarate hydratase from Leishmania major in a complex with fumarate and S-malate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 604 amino acids
Theoretical weight: 66.43 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli
UniProt:
  • Canonical: E9AE57 (Residues: 1-568; Coverage: 100%)
Gene names: FH2, LMJF_29_1960
Sequence domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 65.148Å b: 85.415Å c: 240.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.263
Expression system: Escherichia coli