› 6uqb

X-ray diffraction
1.95Å resolution

Crystal structure of R471A variant of cytosolic fumarate hydratase from Leishmania major in a complex with S-malate and malonate

Released:
DOI: 10.2210/pdb6uqb/pdb
PDB 6uqb coloured by chain and viewed from the front.
PDB 6uqb coloured by chain and viewed from the side.
PDB 6uqb coloured by chain and viewed from the top.
Source organism: Leishmania major strain Friedlin
Primary publication:
Structural and Biochemical Investigations of the [4Fe-4S] Cluster-Containing Fumarate Hydratase from Leishmania major.
Feliciano PR, Drennan CL
Biochemistry 58 5011-5021 (2019)
PMID: 31743022

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 604 amino acids
Theoretical weight: 66.43 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli
UniProt:
  • Canonical: E9AE57 (Residues: 1-568; Coverage: 100%)
Gene names: FH2, LMJF_29_1960
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand SF4 2 x SF4
Ligand LMR 2 x LMR
Ligand MLA 4 x MLA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 65.462Å b: 84.943Å c: 240.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.174
Expression system: Escherichia coli

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