6uph

Electron Microscopy
2.7Å resolution

Structure of a Yeast Centromeric Nucleosome at 2.7 Angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Histone H3-like centromeric protein CSE4 Chains: A, E
Molecule details ›
Chains: A, E
Length: 229 amino acids
Theoretical weight: 26.89 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P36012 (Residues: 1-229; Coverage: 100%)
Gene names: CSE4, CSL2, YKL049C, YKL262
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 118 amino acids
Theoretical weight: 13.33 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CMU6 (Residues: 1-103; Coverage: 100%)
Gene names: KLLA0_E08647g, KLLA0_E17601g
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A Chains: C, G
Molecule details ›
Chains: C, G
Length: 145 amino acids
Theoretical weight: 15.74 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CK59 (Residues: 1-130; Coverage: 100%)
Gene names: HTA1, HTA2, KLLA0E17413g, KLLA0F13332g
Sequence domains:
Histone H2B.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 147 amino acids
Theoretical weight: 16.22 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CK60 (Residues: 1-132; Coverage: 100%)
Gene names: HTB1, KLLA0F13310g
Sequence domains: Core histone H2A/H2B/H3/H4
DNA (119-MER) Chain: I
Molecule details ›
Chain: I
Length: 147 nucleotides
Theoretical weight: 45.14 KDa
DNA (119-MER) Chain: J
Molecule details ›
Chain: J
Length: 147 nucleotides
Theoretical weight: 45.61 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-20839
Expression system: Escherichia coli