› 6un2

X-ray diffraction
0.97Å resolution

Crystal structure of photoactive yellow protein (PYP); C69U construct (selenocysteine incorporation)

Released:
DOI: 10.2210/pdb6un2/pdb
PDB 6un2 coloured by chain and viewed from the front.
PDB 6un2 coloured by chain and viewed from the side.
PDB 6un2 coloured by chain and viewed from the top.
Source organism: Halorhodospira halophila
Primary publication:
Structural and spectroscopic characterization of photoactive yellow protein and photoswitchable fluorescent protein constructs containing heavy atoms.
Romei MG, Lin CY, Boxer SG
J Photochem Photobiol A Chem 401 (2020)
PMID: 32753830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 14.22 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
1 modified residue:
Ligand SEC 1 x SEC

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P63
Unit cell:
a: 65.859Å b: 65.859Å c: 40.531Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.135 0.134 0.157
Expression system: Escherichia coli BL21(DE3)

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