6ukf

X-ray diffraction
1Å resolution

HhaI endonuclease in Complex with DNA at 1 Angstrom Resolution

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HhaI Restriction Endonuclease Chain: X
Molecule details ›
Chain: X
Length: 258 amino acids
Theoretical weight: 29.98 KDa
Source organism: Haemophilus parahaemolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: I3DBY6 (Residues: 1-258; Coverage: 100%)
Gene name: HMPREF1050_0931
DNA (5'-D(*TP*CP*CP*AP*AP*GP*CP*GP*CP*AP*AP*CP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Haemophilus parahaemolyticus
Expression system: Not provided
DNA (5'-D(*CP*GP*(5IT)P*TP*GP*CP*GP*CP*TP*(5IT)P*GP*GP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.22 KDa
Source organism: Haemophilus parahaemolyticus
Expression system: Not provided

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 61.018Å b: 37.317Å c: 69.027Å
α: 90° β: 109.768° γ: 90°
R-values:
R R work R free
0.155 0.155 0.166
Expression systems:
  • Escherichia coli
  • Not provided