6uek

X-ray diffraction
2.16Å resolution

Structure of Urocanate Hydratase from Trypanosoma cruzi in complex with NAD+

Released:
Source organism: Trypanosoma cruzi
Primary publication:
Structure of Urocanate Hydratase from the protozoan Trypanosoma cruzi.
Int J Biol Macromol 146 716-724 (2020)
PMID: 31843618

Function and Biology Details

Reaction catalysed:
3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urocanate hydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 681 amino acids
Theoretical weight: 75.69 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4D9S6 (Residues: 1-675; Coverage: 100%)
Gene name: Tc00.1047053504045.110
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21
Unit cell:
a: 84.614Å b: 134.788Å c: 113.399Å
α: 90° β: 93.25° γ: 90°
R-values:
R R work R free
0.218 0.218 0.255
Expression system: Escherichia coli