› 6ubr

X-ray diffraction
1.96Å resolution

Crystal structure of D678A GoxA bound to glycine at pH 7.5

Released:
DOI: 10.2210/pdb6ubr/pdb
PDB 6ubr coloured by chain and viewed from the front.
PDB 6ubr coloured by chain and viewed from the side.
PDB 6ubr coloured by chain and viewed from the top.
Source organism: Pseudoalteromonas luteoviolacea DSM 6061
Primary publication:
Kinetic and structural evidence that Asp-678 plays multiple roles in catalysis by the quinoprotein glycine oxidase.
Mamounis KJ, Avalos D, Yukl ET, Davidson VL
J Biol Chem 294 17463-17470 (2019)
PMID: 31615898

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 816 amino acids
Theoretical weight: 91.48 KDa
Source organism: Pseudoalteromonas luteoviolacea DSM 6061
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A161XU12 (Residues: 1-816; Coverage: 100%)
Gene name: N475_19905
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand SO4 2 x SO4
Ligand PEG 1 x PEG
Ligand GLY 4 x GLY
1 modified residue:
Ligand TRQ 4 x TRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 110.679Å b: 93.348Å c: 187.853Å
α: 90° β: 94.95° γ: 90°
R-values:
R R work R free
0.176 0.175 0.209
Expression system: Escherichia coli

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Citations

2 citation in other articles

Functional and structural characterization of a flavoprotein monooxygenase essential for biogenesis of tryptophylquinone cofactor.
Oozeki et al. (2021)
1 more