6uaz

X-ray diffraction
1.85Å resolution

Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III) in complex with glucose

Released:
Source organism: Blastomyces gilchristii
Entry authors: Costa PACR, Santos CR, Domingues MN, Lima EA, Mandelli F, Murakami MT

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyco_hydro_cc domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 27.99 KDa
Source organism: Blastomyces gilchristii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A179UGT5 (Residues: 23-262; Coverage: 100%)
Gene name: BDBG_03339
Sequence domains: Glycosyl hydrolase catalytic core

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P1
Unit cell:
a: 36.46Å b: 48.928Å c: 57.757Å
α: 102.97° β: 89.34° γ: 93.76°
R-values:
R R work R free
0.167 0.164 0.217
Expression system: Escherichia coli