6u9d

X-ray diffraction
3.19Å resolution

Saccharomyces cerevisiae acetohydroxyacid synthase

Released:

Function and Biology Details

Reaction catalysed:
2 pyruvate = 2-acetolactate + CO(2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acetolactate synthase catalytic subunit, mitochondrial Chains: A, B, E, F, I, J, M, N, Q, R, U, V
Molecule details ›
Chains: A, B, E, F, I, J, M, N, Q, R, U, V
Length: 644 amino acids
Theoretical weight: 70.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P07342 (Residues: 58-687; Coverage: 92%)
Gene names: ILV2, SMR1, YM9718.07, YMR108W
Sequence domains:
Acetolactate synthase small subunit, mitochondrial Chains: C, D, G, H, K, L, O, P, S, T, W, X
Molecule details ›
Chains: C, D, G, H, K, L, O, P, S, T, W, X
Length: 297 amino acids
Theoretical weight: 32.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25605 (Residues: 41-309; Coverage: 87%)
Gene names: ILV6, YCL009C, YCL9C
Sequence domains:

Ligands and Environments


Cofactor: Ligand TPP 11 x TPP

Cofactor: Ligand FAD 12 x FAD
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 368.647Å b: 230.307Å c: 183.532Å
α: 90° β: 94.57° γ: 90°
R-values:
R R work R free
0.205 0.205 0.252
Expression system: Escherichia coli