6u62

Electron Microscopy
3.18Å resolution

Raptor-Rag-Ragulator complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Regulatory-associated protein of mTOR Chain: A
Molecule details ›
Chain: A
Length: 1392 amino acids
Theoretical weight: 154.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N122 (Residues: 1-1335; Coverage: 100%)
Gene names: KIAA1303, RAPTOR, RPTOR
Sequence domains:
Ras-related GTP-binding protein A Chain: B
Molecule details ›
Chain: B
Length: 313 amino acids
Theoretical weight: 36.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7L523 (Residues: 1-313; Coverage: 100%)
Gene name: RRAGA
Sequence domains: Gtr1/RagA G protein conserved region
Ras-related GTP-binding protein C Chain: C
Molecule details ›
Chain: C
Length: 400 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HB90 (Residues: 2-399; Coverage: 100%)
Gene name: RRAGC
Sequence domains: Gtr1/RagA G protein conserved region
Ragulator complex protein LAMTOR1 Chain: D
Molecule details ›
Chain: D
Length: 158 amino acids
Theoretical weight: 17.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6IAA8 (Residues: 6-161; Coverage: 97%)
Gene names: C11orf59, LAMTOR1, PDRO, PP7157
Sequence domains: Late endosomal/lysosomal adaptor and MAPK and MTOR activator
Ragulator complex protein LAMTOR2 Chain: E
Molecule details ›
Chain: E
Length: 125 amino acids
Theoretical weight: 13.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y2Q5 (Residues: 1-125; Coverage: 100%)
Gene names: HSPC003, LAMTOR2, MAPBPIP, ROBLD3
Sequence domains: Roadblock/LC7 domain
Ragulator complex protein LAMTOR3 Chain: F
Molecule details ›
Chain: F
Length: 162 amino acids
Theoretical weight: 17.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHA4 (Residues: 1-124; Coverage: 100%)
Gene names: LAMTOR3, MAP2K1IP1, MAPKSP1, PRO2783
Sequence domains: Mitogen-activated protein kinase kinase 1 interacting
Ragulator complex protein LAMTOR4 Chain: G
Molecule details ›
Chain: G
Length: 100 amino acids
Theoretical weight: 10.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0VGL1 (Residues: 2-99; Coverage: 99%)
Gene names: C7orf59, LAMTOR4
Ragulator complex protein LAMTOR5 Chain: H
Molecule details ›
Chain: H
Length: 91 amino acids
Theoretical weight: 9.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43504 (Residues: 1-91; Coverage: 100%)
Gene names: HBXIP, LAMTOR5, XIP
Sequence domains: Ragulator complex protein LAMTOR5

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.18Å
Relevant EMDB volumes: EMD-20660
Expression systems:
  • Homo sapiens
  • Escherichia coli