6u42

Electron Microscopy
3.4Å resolution

Natively decorated ciliary doublet microtubule

Released:
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structure of the Decorated Ciliary Doublet Microtubule.
Cell 179 909-922.e12 (2019)
PMID: 31668805
Related structures: EMD-20631

Function and Biology Details

Reaction catalysed:
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 451-mer (preferred)
Entry contents:
38 distinct polypeptide molecules
Macromolecules (38 distinct):
Tubulin beta-1/beta-2 chain Chains: 0A, 0C, 0E, 0G, 0I, 0K, 0M, 0O, 0Q, 0S, 0U, 0W, 0Y, 1, 1A, 1C, 1E, 1G, 1K, 1M, 1O, 1Q, 1S, 1U, 1W, 2A, 2C, 2E, 2G, 2I, 2K, 2M, 2Q, 2S, 2U, 2W, 2Y, 3, 3A, 3C, 3G, 3I, 3K, 3M, 3O, 3Q, 3S, 5, 7, 9, A1, A3, A5, A7, A9, B1, B3, B5, B8, C0, C2, C4, C6, C8, D0, D2, D4, D6, D8, E0, E2, E4, E6, E8, F0, F2, F6, F8, G0, G2, G4, G6, G8, H2, H4, H6, H8, I0, I2, I4, I8, J0, J2, J4, J6, J8, K0, K4, K6, K8, L0, L2, L4, L6, M0, M2, M4, M6, M8, N0, N2, N6, N8, O0, O2, O4, O6, O8, P4, P6, P8, Q0, Q2, Q4, Q6, Q8, R0, R2, R4, R6, R8, S0, S2, S4, S6, S8, T0, T2, T4, T6, T8, U2, U4, U6, U8, V0, V2, V4, V6, V8, W0, W2, W4, W6, W8, X0, X2, X4, X6, X8, Y0, Y2, Y4, Y6, Y8, Z0, Z2, Z4, Z6, Z8
Molecule details ›
Chains: 0A, 0C, 0E, 0G, 0I, 0K, 0M, 0O, 0Q, 0S, 0U, 0W, 0Y, 1, 1A, 1C, 1E, 1G, 1K, 1M, 1O, 1Q, 1S, 1U, 1W, 2A, 2C, 2E, 2G, 2I, 2K, 2M, 2Q, 2S, 2U, 2W, 2Y, 3, 3A, 3C, 3G, 3I, 3K, 3M, 3O, 3Q, 3S, 5, 7, 9, A1, A3, A5, A7, A9, B1, B3, B5, B8, C0, C2, C4, C6, C8, D0, D2, D4, D6, D8, E0, E2, E4, E6, E8, F0, F2, F6, F8, G0, G2, G4, G6, G8, H2, H4, H6, H8, I0, I2, I4, I8, J0, J2, J4, J6, J8, K0, K4, K6, K8, L0, L2, L4, L6, M0, M2, M4, M6, M8, N0, N2, N6, N8, O0, O2, O4, O6, O8, P4, P6, P8, Q0, Q2, Q4, Q6, Q8, R0, R2, R4, R6, R8, S0, S2, S4, S6, S8, T0, T2, T4, T6, T8, U2, U4, U6, U8, V0, V2, V4, V6, V8, W0, W2, W4, W6, W8, X0, X2, X4, X6, X8, Y0, Y2, Y4, Y6, Y8, Z0, Z2, Z4, Z6, Z8
Length: 443 amino acids
Theoretical weight: 49.67 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P04690 (Residues: 1-443; Coverage: 100%)
Gene names: TUBB1, TUBB2
Sequence domains:
Tubulin alpha-1 chain Chains: 0B, 0F, 0H, 0J, 0L, 0N, 0P, 0R, 0V, 0X, 0Z, 1B, 1D, 1F, 1H, 1L, 1N, 1P, 1R, 1T, 1V, 1X, 1Z, 2, 2B, 2D, 2F, 2H, 2J, 2L, 2N, 2P, 2R, 2T, 2V, 2X, 2Z, 3B, 3D, 3F, 3H, 3J, 3L, 3N, 3P, 3R, 3T, 4, 6, 8, A0, A2, A4, A8, B0, B2, B4, B7, B9, C1, C5, C7, C9, D1, D3, D5, D7, E1, E3, E5, E7, E9, F1, F3, F5, F7, F9, G1, G3, G5, G7, G9, H1, H3, H5, H7, H9, I1, I3, I5, I7, I9, J1, J3, J5, J7, J9, K1, K3, K5, K7, K9, L1, L3, L5, L7, L9, M1, M3, M5, M7, M9, N1, N3, N5, N7, N9, O1, O3, O5, O7, O9, P3, P5, P7, P9, Q1, Q3, Q5, Q9, R1, R3, R5, R7, R9, S1, S5, S7, S9, T1, T3, T5, T7, U1, U3, U5, U7, U9, V1, V3, V7, V9, W1, W3, W5, W7, W9, X3, X5, X7, X9, Y1, Y3, Y5, Y9, Z1, Z3, Z5, Z7, Z9
Molecule details ›
Chains: 0B, 0F, 0H, 0J, 0L, 0N, 0P, 0R, 0V, 0X, 0Z, 1B, 1D, 1F, 1H, 1L, 1N, 1P, 1R, 1T, 1V, 1X, 1Z, 2, 2B, 2D, 2F, 2H, 2J, 2L, 2N, 2P, 2R, 2T, 2V, 2X, 2Z, 3B, 3D, 3F, 3H, 3J, 3L, 3N, 3P, 3R, 3T, 4, 6, 8, A0, A2, A4, A8, B0, B2, B4, B7, B9, C1, C5, C7, C9, D1, D3, D5, D7, E1, E3, E5, E7, E9, F1, F3, F5, F7, F9, G1, G3, G5, G7, G9, H1, H3, H5, H7, H9, I1, I3, I5, I7, I9, J1, J3, J5, J7, J9, K1, K3, K5, K7, K9, L1, L3, L5, L7, L9, M1, M3, M5, M7, M9, N1, N3, N5, N7, N9, O1, O3, O5, O7, O9, P3, P5, P7, P9, Q1, Q3, Q5, Q9, R1, R3, R5, R7, R9, S1, S5, S7, S9, T1, T3, T5, T7, U1, U3, U5, U7, U9, V1, V3, V7, V9, W1, W3, W5, W7, W9, X3, X5, X7, X9, Y1, Y3, Y5, Y9, Z1, Z3, Z5, Z7, Z9
Length: 451 amino acids
Theoretical weight: 49.64 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P09204 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1
Sequence domains:
Parkin-co-regulated gene product Chains: 3V, 3X, 3Z, 4B, 4D, 4F, 4H, 4J
Molecule details ›
Chains: 3V, 3X, 3Z, 4B, 4D, 4F, 4H, 4J
Length: 307 amino acids
Theoretical weight: 34.22 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: B1B601 (Residues: 1-307; Coverage: 100%)
Gene names: CHLRE_02g113400v5, PACRG
Cilia- and flagella-associated protein 20 Chains: 3W, 3Y, 4A, 4C, 4E, 4G, 4I
Molecule details ›
Chains: 3W, 3Y, 4A, 4C, 4E, 4G, 4I
Length: 190 amino acids
Theoretical weight: 22.19 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IU92 (Residues: 1-190; Coverage: 100%)
Gene names: BUG22, CFAP20, CHLREDRAFT_189631, FAP20
Sequence domains: Protein of unknown function (DUF667)
Cilia- and flagella-associated protein 53 Chains: 4L, 4M
Molecule details ›
Chains: 4L, 4M
Length: 482 amino acids
Theoretical weight: 56.66 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IRJ7 (Residues: 1-482; Coverage: 100%)
Gene names: CFAP53, CHLREDRAFT_196792, FAP53
Sequence domains: Trichohyalin-plectin-homology domain
Meiosis-specific nuclear structural protein 1 Chains: 4N, 4O, 4P
Molecule details ›
Chains: 4N, 4O, 4P
Length: 505 amino acids
Theoretical weight: 60.03 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3CRG7 (Residues: 1-505; Coverage: 100%)
Gene name: CHLRE_17g737100v5
Sequence domains: Trichohyalin-plectin-homology domain
Coiled-coil protein associated with protofilament ribbons Chains: 4Q, 4R, 4S, 4T, 4U
Molecule details ›
Chains: 4Q, 4R, 4S, 4T, 4U
Length: 367 amino acids
Theoretical weight: 42.71 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q9M6B0 (Residues: 1-367; Coverage: 100%)
Gene names: CHLREDRAFT_77703, RIB43a
Flagellar associated protein Chains: 4V, 4W, 4X
Molecule details ›
Chains: 4V, 4W, 4X
Length: 475 amino acids
Theoretical weight: 53.13 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8J0X0 (Residues: 1-475; Coverage: 100%)
Gene names: CHLREDRAFT_174030, CHLRE_02g145850v5, FAP112
TPH domain-containing protein Chains: 4Y, 4Z
Molecule details ›
Chains: 4Y, 4Z
Length: 462 amino acids
Theoretical weight: 53.64 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3E0E3 (Residues: 1-462; Coverage: 100%)
Gene name: CHLRE_02g078550v5
Sequence domains: Trichohyalin-plectin-homology domain
Cilia- and flagella-associated protein 45 Chains: 5A, 5B, 5C, 5D
Molecule details ›
Chains: 5A, 5B, 5C, 5D
Length: 501 amino acids
Theoretical weight: 59.04 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8I9E8 (Residues: 1-501; Coverage: 100%)
Gene names: CFAP45, CHLREDRAFT_206096, FAP45
Sequence domains: Trichohyalin-plectin-homology domain
RIB30 Chain: 5E
Molecule details ›
Chain: 5E
Length: 274 amino acids
Theoretical weight: 29.86 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3E5X9 (Residues: 1-274; Coverage: 100%)
Gene name: CHLRE_01g017650v5
FAP363 Chains: 5F, 5G, 7B
Molecule details ›
Chains: 5F, 5G, 7B
Length: 391 amino acids
Theoretical weight: 43.88 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IY81 (Residues: 1-391; Coverage: 100%)
Gene names: CHLREDRAFT_190810, CHLRE_12g545850v5
Protein Flattop homolog Chains: 5H, 5I, 5J, 5K
Molecule details ›
Chains: 5H, 5I, 5J, 5K
Length: 137 amino acids
Theoretical weight: 15.44 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IVJ1 (Residues: 1-137; Coverage: 100%)
Gene names: CFAP126, CHLREDRAFT_190291, FAP126, FLTP
FAP52 Chains: 5L, 5M, 5N
Molecule details ›
Chains: 5L, 5M, 5N
Length: 633 amino acids
Theoretical weight: 68.55 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3D260 (Residues: 1-633; Coverage: 100%)
Gene name: CHLRE_12g489750v5
Sequence domains: WD domain, G-beta repeat
Nucleoside diphosphate kinase Chains: 5O, 5P
Molecule details ›
Chains: 5O, 5P
Length: 380 amino acids
Theoretical weight: 41.99 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JGW5 (Residues: 1-380; Coverage: 100%)
Gene names: CHLREDRAFT_180221, CHLRE_12g558700v5, FAP67
Sequence domains: Nucleoside diphosphate kinase
EF-hand domain-containing protein Chain: 5Q
Molecule details ›
Chain: 5Q
Length: 197 amino acids
Theoretical weight: 22.23 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8J250 (Residues: 1-197; Coverage: 100%)
Gene names: CHLREDRAFT_191679, CHLRE_06g283100v5, FAP85
Enkurin domain-containing protein Chains: 5R, 5S, 5T, 5U, 5V
Molecule details ›
Chains: 5R, 5S, 5T, 5U, 5V
Length: 240 amino acids
Theoretical weight: 27.02 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8J098 (Residues: 1-240; Coverage: 100%)
Gene names: CHLREDRAFT_137793, CHLRE_09g393954v5, FAP106
Sequence domains: Calmodulin-binding
Flagellar associated protein Chains: 5X, 5Y, 5Z, 6A, 6B, 6C, 6D
Molecule details ›
Chains: 5X, 5Y, 5Z, 6A, 6B, 6C, 6D
Length: 230 amino acids
Theoretical weight: 26.65 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JDK1 (Residues: 1-230; Coverage: 100%)
Gene names: CHLREDRAFT_194105, CHLRE_02g142126v5, FAP115
FAP143 Chain: 6E
Molecule details ›
Chain: 6E
Length: 268 amino acids
Theoretical weight: 29.22 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3E1X6 (Residues: 1-268; Coverage: 100%)
Gene name: CHLRE_02g095149v5
Flagellar associated protein Chain: 6F
Molecule details ›
Chain: 6F
Length: 403 amino acids
Theoretical weight: 42.85 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8HRH3 (Residues: 1-403; Coverage: 100%)
Gene names: CHLREDRAFT_156094, CHLRE_13g564400v5, FAP161
FAP166 Chains: 6G, 6H
Molecule details ›
Chains: 6G, 6H
Length: 214 amino acids
Theoretical weight: 23.3 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3DZF0 (Residues: 1-214; Coverage: 100%)
Gene name: CHLRE_03g200700v5
Flagellar associated protein Chains: 6I, 6J, 6K, 6L, 6M, 6N, 6O, 6P
Molecule details ›
Chains: 6I, 6J, 6K, 6L, 6M, 6N, 6O, 6P
Length: 352 amino acids
Theoretical weight: 39.51 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8I1S7 (Residues: 1-352; Coverage: 100%)
Gene names: CHLREDRAFT_187818, CHLRE_10g466650v5, FAP252
Sequence domains: EF-hand domain
FAP276 Chains: 6Q, 6R, 6S
Molecule details ›
Chains: 6Q, 6R, 6S
Length: 87 amino acids
Theoretical weight: 10.07 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3DTN6 (Residues: 1-87; Coverage: 100%)
Gene names: CHLRE_04g216203v5, CHLRE_04g216250v5
p72 Chains: 6T, 6U, 6V, 6W, 6X, 6Y, 6Z, 7A
Molecule details ›
Chains: 6T, 6U, 6V, 6W, 6X, 6Y, 6Z, 7A
Length: 635 amino acids
Theoretical weight: 72.08 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q8LKK4 (Residues: 1-635; Coverage: 100%)
Gene names: CHLREDRAFT_126286, CHLRE_02g091700v5, RIB72
Sequence domains: DUF1126 PH-like domain
Flagellar associated protein Chain: 7C
Molecule details ›
Chain: 7C
Length: 111 amino acids
Theoretical weight: 12.74 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IMR3 (Residues: 1-111; Coverage: 100%)
Gene names: CHLREDRAFT_170025, FAP141
Sequence domains: Domain of unknown function (DUF4558)
Flagellar associated protein Chains: 7D, 7E, 7F
Molecule details ›
Chains: 7D, 7E, 7F
Length: 146 amino acids
Theoretical weight: 15.92 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JF23 (Residues: 1-146; Coverage: 100%)
Gene names: CHLREDRAFT_153689, CHLRE_11g467561v5, FAP222
Flagellar associated protein Chain: 7G
Molecule details ›
Chain: 7G
Length: 235 amino acids
Theoretical weight: 25.58 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8HSW0 (Residues: 1-235; Coverage: 100%)
Gene names: CHLREDRAFT_182707, CHLRE_13g573500v5, FAP95
Flagellar associated protein Chains: 7H, 7X
Molecule details ›
Chains: 7H, 7X
Length: 471 amino acids
Theoretical weight: 48.84 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JHR6 (Residues: 1-471; Coverage: 100%)
Gene names: CHLREDRAFT_195050, CHLRE_12g485000v5, FAP182
FAP129 Chains: 7I, 7J
Molecule details ›
Chains: 7I, 7J
Length: 483 amino acids
Theoretical weight: 51.33 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3DZI2 (Residues: 1-483; Coverage: 100%)
Gene name: CHLRE_03g199500v5
FAP21 Chains: 7K, 7L
Molecule details ›
Chains: 7K, 7L
Length: 458 amino acids
Theoretical weight: 49.66 KDa
Source organism: Chlamydomonas reinhardtii
Flagellar associated protein Chains: 7M, 7N
Molecule details ›
Chains: 7M, 7N
Length: 139 amino acids
Theoretical weight: 15.47 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IXN7 (Residues: 1-139; Coverage: 100%)
Gene names: CHLREDRAFT_183955, CHLRE_03g200950v5, FAP273
Flagellar associated protein Chains: 7O, 7P
Molecule details ›
Chains: 7O, 7P
Length: 238 amino acids
Theoretical weight: 25.94 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JC52 (Residues: 1-238; Coverage: 100%)
Gene names: CHLREDRAFT_178177, CHLRE_11g469000v5, FAP107
RIB21 Chain: 7Q
Molecule details ›
Chain: 7Q
Length: 189 amino acids
Theoretical weight: 20.95 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A0A2K3D7C7 (Residues: 1-189; Coverage: 100%)
Gene name: CHLRE_11g467582v5
Outer dynein arm-docking complex subunit 1 Chains: 7R, 7S
Molecule details ›
Chains: 7R, 7S
Length: 749 amino acids
Theoretical weight: 83.52 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IPZ5 (Residues: 1-749; Coverage: 100%)
Gene names: CHLREDRAFT_189083, CHLRE_17g703850v5, ODA3
Outer dynein arm protein 1 Chains: 7T, 7U
Molecule details ›
Chains: 7T, 7U
Length: 552 amino acids
Theoretical weight: 62.29 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8JF70 (Residues: 1-552; Coverage: 100%)
Gene names: CHLREDRAFT_132719, DCC2, ODA1
Outer dynein arm-docking complex subunit 3 Chains: 7V, 7W
Molecule details ›
Chains: 7V, 7W
Length: 184 amino acids
Theoretical weight: 21.37 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q7Y0H2 (Residues: 1-184; Coverage: 100%)
Gene names: CHLREDRAFT_136411, CHLRE_14g617550v5, ODA14
Flagellar associated protein Chain: 7Y
Molecule details ›
Chain: 7Y
Length: 312 amino acids
Theoretical weight: 35.57 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8HPK6 (Residues: 1-312; Coverage: 100%)
Gene names: CHLREDRAFT_146275, CHLRE_01g015850v5, FAP68
Flagellar associated protein Chains: 7Z, 8A, 8B, 8C, 8D
Molecule details ›
Chains: 7Z, 8A, 8B, 8C, 8D
Length: 242 amino acids
Theoretical weight: 27.76 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8ICC1 (Residues: 1-242; Coverage: 100%)
Gene names: CHLREDRAFT_181900, CHLRE_10g424250v5, FAP90

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-20631