X-ray diffraction
1.69Å resolution

Function and Biology Details

Reaction catalysed:
(1a) ATP + [RNA ligase]-L-lysine = [RNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 49.54 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: P43075 (Residues: 401-832; Coverage: 52%)
Gene names: CAALFM_C702060WA, CaJ7.0238, CaO19.13864, CaO19.6511, LIG1, RLG1, TRL1
Sequence domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 50.141Å b: 56.427Å c: 82.501Å
α: 90° β: 90° γ: 90°
R R work R free
0.183 0.18 0.209
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'