6tzc

X-ray diffraction
2.41Å resolution

Crystal Structure of African Swine Fever Virus A179L with the Autophagy Regulator Beclin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 40.68 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Apoptosis regulator Bcl-2 homolog Chain: B
Molecule details ›
Chain: B
Length: 154 amino acids
Theoretical weight: 18.13 KDa
Source organism: African swine fever virus BA71V
Expression system: Escherichia coli
UniProt:
  • Canonical: P42485 (Residues: 1-148; Coverage: 83%)
Gene names: A179L, Ba71V-041
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Beclin-1 Chain: C
Molecule details ›
Chain: C
Length: 26 amino acids
Theoretical weight: 2.85 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: Q4A1L5 (Residues: 103-128; Coverage: 6%)
Gene names: ATG6, BECN1
Sequence domains: Beclin-1 BH3 domain, Bcl-2-interacting

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 54.565Å b: 44.347Å c: 129.022Å
α: 90° β: 94.532° γ: 90°
R-values:
R R work R free
0.216 0.214 0.253
Expression systems:
  • Escherichia coli
  • Not provided