Protein Data Bank in Europe

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.

RX + glutathione = HX + R-S-glutathione

ATP + L-arginine = ADP + N(omega)-phospho-L-arginine

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin

Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA

Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic

(S)-lactate + NAD(+) = pyruvate + NADH

(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine

(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine

4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH

L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH

Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

Peptidylproline (omega=180) = peptidylproline (omega=0)

L-arginine + H(2)O = L-ornithine + urea

A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]

DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine

NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O

Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.

Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)

NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)

Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-

Cutin + H(2)O = cutin monomers

NTP + H(2)O = NDP + phosphate

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate

2 H(2)O(2) = O(2) + 2 H(2)O

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)

Choline = trimethylamine + acetaldehyde

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

Release of N-terminal proline from a peptide.

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans

ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)

Succinate semialdehyde + NADP(+) + H(2)O = succinate + NADPH

(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O

N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)

(1a) NAD(+) = cyclic ADP-ribose + nicotinamide

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate

dUTP + H(2)O = dUMP + diphosphate

Cleavage of peptide bonds with very broad specificity.

Chorismate = prephenate

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

Hydroxymethylbilane = uroporphyrinogen III + H(2)O

ATP + H(2)O = ADP + phosphate

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH

A beta-lactam + H(2)O = a substituted beta-amino acid

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH

A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)

5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H