X-ray diffraction
1.15Å resolution

Human Aldose Reductase in complex with ALR43

Source organism: Homo sapiens
Entry authors: Sandner A, Heine A, Klebe G

Function and Biology Details

Reactions catalysed:
All-trans-retinol + NADP(+) = all-trans-retinal + NADPH
Alditol + NAD(P)(+) = aldose + NAD(P)H
Allyl alcohol + NADP(+) = acrolein + NADPH
Glycerol + NADP(+) = D-glyceraldehyde + NADPH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Aldo-keto reductase family 1 member B1 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 35.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P15121 (Residues: 1-316; Coverage: 100%)
Gene names: AKR1B1, ALDR1, ALR2
Sequence domains: Aldo/keto reductase family

Ligands and Environments

Cofactor: Ligand NAP 1 x NAP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 47.175Å b: 66.44Å c: 49.219Å
α: 90° β: 92.395° γ: 90°
R R work R free
0.13 0.129 0.152
Expression system: Escherichia coli BL21(DE3)