› 6tqv

X-ray diffraction
1.35Å resolution

Crystal structure of ribonucleotide reductase NrdF from Bacillus anthracis aerobically soaked with Fe(II) and Mn(II) ions

Released:
DOI: 10.2210/pdb6tqv/pdb
PDB entry 6tqv coloured by chain, front view.
PDB entry 6tqv coloured by chain, side view.
PDB entry 6tqv coloured by chain, top view.
Source organism: Bacillus anthracis str. Sterne
Primary publication:
The Bacillus anthracis class Ib ribonucleotide reductase subunit NrdF intrinsically selects manganese over iron.
Grāve K, Griese JJ, Berggren G, Bennett MD, Högbom M
J Biol Inorg Chem 25 571-582 (2020)
PMID: 32296998

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 37.06 KDa
Source organism: Bacillus anthracis str. Sterne
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81TB4 (Residues: 1-322; Coverage: 100%)
Gene names: GBAA_1372, nrdF
Sequence domains: Ribonucleotide reductase, small chain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 57.178Å b: 60.3Å c: 95.831Å
α: 90° β: 106.469° γ: 90°
R-values:
R R work R free
0.148 0.147 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Metalation: nature's challenge in bioinorganic chemistry.
Robinson et al. (2020)
4 more

1 mention without citation

The Bacillus anthracis class Ib ribonucleotide reductase subunit NrdF intrinsically selects manganese over iron.
Grāve et al. (2020)