› 6tqr

X-ray diffraction
1.85Å resolution

The crystal structure of the MSP domain of human VAP-A in complex with the Phospho-FFAT motif of STARD3.

Released:
DOI: 10.2210/pdb6tqr/pdb
PDB entry 6tqr coloured by chain, front view.
PDB entry 6tqr coloured by chain, side view.
PDB entry 6tqr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
FFAT motif phosphorylation controls formation and lipid transfer function of inter-organelle contacts.
Di Mattia T, Martinet A, Ikhlef S, McEwen AG, Nominé Y, Wendling C, Poussin-Courmontagne P, Voilquin L, Eberling P, Ruffenach F, Cavarelli J, Slee J, Levine TP, Drin G, Tomasetto C, Alpy F
EMBO J 39 e104369 (2020)
PMID: 33124732

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172114 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vesicle-associated membrane protein-associated protein A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 213 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P0L0 (Residues: 8-212; Coverage: 82%)
Gene names: VAP33, VAPA
Sequence domains: MSP (Major sperm protein) domain
StAR-related lipid transfer protein 3 Chains: E, F
Molecule details ›
Chains: E, F
Length: 17 amino acids
Theoretical weight: 1.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q14849 (Residues: 200-216; Coverage: 4%)
Gene names: CAB1, MLN64, STARD3

Ligands and Environments

1 bound ligand:
Ligand CL 7 x CL
1 modified residue:
Ligand SEP 6 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 39.14Å b: 43.78Å c: 83.64Å
α: 89.46° β: 92.91° γ: 105.13°
R-values:
R R work R free
0.207 0.204 0.254
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

19 review citations

The Vps13 Family of Lipid Transporters and Its Role at Membrane Contact Sites.
Dziurdzik et al. (2021)
18 more

2 mentions without citation

The Interactome of the VAP Family of Proteins: An Overview.
James et al. (2021)
1 more