› 6tpc

X-ray diffraction
1.52Å resolution

Crystal structure of Endoglucanase N194A from Penicillium verruculosum

Released:
DOI: 10.2210/pdb6tpc/pdb
PDB entry 6tpc coloured by chain, front view.
PDB entry 6tpc coloured by chain, side view.
PDB entry 6tpc coloured by chain, top view.
Source organism: Talaromyces verruculosus
Entry authors: Nemashkalov V, Kravchenko O, Gabdulkhakov A, Tischenko S, Rozhkova A, Sinitsyn A

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
cellulase Chains: A, C
Molecule details ›
Chains: A, C
Length: 314 amino acids
Theoretical weight: 34.6 KDa
Source organism: Talaromyces verruculosus
Expression system: Penicillium canescens
UniProt:
  • Canonical: A0A1U7Q1U3 (Residues: 1-305; Coverage: 100%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand SO4 1 x SO4
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Unit cell:
a: 80.372Å b: 82.551Å c: 90.935Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.222
Expression system: Penicillium canescens

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