6tp8

X-ray diffraction
1.55Å resolution

Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B

Released:

Function and Biology Details

Reaction catalysed:
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 317 amino acids
Theoretical weight: 33.04 KDa
Source organism: Moesziomyces antarcticus
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P41365 (Residues: 26-342; Coverage: 98%)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: C2221
Unit cell:
a: 89.426Å b: 156.654Å c: 138.084Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.192
Expression system: Aspergillus oryzae